{"id":4397,"date":"2020-09-22T09:07:43","date_gmt":"2020-09-22T06:07:43","guid":{"rendered":"http:\/\/drakopoulos-ivf.gr\/?page_id=4397"},"modified":"2025-12-09T12:49:13","modified_gmt":"2025-12-09T10:49:13","slug":"ivf-icsi","status":"publish","type":"page","link":"http:\/\/drakopoulos-ivf.gr\/en\/ivf-icsi\/","title":{"rendered":"In Vitro Fertilisation (IVF)"},"content":{"rendered":"
[mk_page_section bg_image=”https:\/\/drakopoulos-ivf.gr\/wp-content\/uploads\/2020\/09\/-\u03b6\u03b5\u03c5\u03b3\u03b1\u03c1\u03b9\u03bf\u03cd-e1600969473480.jpg” bg_position=”center center” bg_stretch=”true” video_color_mask=”#0a0a0a” video_opacity=”0.4″ sidebar=”sidebar-1″][vc_column][vc_column_text css=”.vc_custom_1731610011284{margin-bottom: 0px !important;padding-top: 130px !important;padding-bottom: 120px !important;}”]\n

In Vitro Fertilisation (IVF)<\/span><\/h1>\n

How is IVF done? \u2192 Ovarian stimulation \u2192 Ovulation \u2192 Fertilization \u2192 Fertilization \u2192 Embryo transfer<\/span><\/p>\n[\/vc_column_text][\/vc_column][\/mk_page_section][vc_row css=”.vc_custom_1731609343964{padding-top: 45px !important;padding-bottom: 10px !important;}”][vc_column][vc_column_text]IVF<\/strong> is the joining of eggs and sperm in the laboratory – extracorporeally – in order to produce fertilised embryos for transfer to the uterus of the expectant mother.
\nThe fertilisation of eggs<\/strong>can be carried out either by means of the conventional IVF method (IVF) or by means of the microfertilisation method (ICSI).<\/p>\n

IVF with the couple’s own sperm<\/strong> is recommended in:<\/p>\n

    \n
  • Couples for whom simple methods, such as Intrauterine Insemination (IUI), have failed.<\/li>\n
  • Women with complete absence or damage to the fallopian tubes.<\/li>\n
  • Women suffering from advanced endometriosis, with possible complications in the fallopian tubes and egg quality.<\/li>\n
  • Situations where there is a limited number of eggs.<\/li>\n
  • Cases of severe male infertility.<\/li>\n<\/ul>\n

    IVF with donor sperm<\/strong> is recommended in:<\/p>\n

      \n
    • Severe cases of male infertility, with poor sperm quality.<\/li>\n
    • Previous failure to inseminate with sperm from the couple.<\/li>\n
    • Cases of poor embryo quality or repeated implantation failures, mainly due to the male factor.<\/li>\n
    • Male carriers of a genetic disease that cannot be studied in embryos.<\/li>\n
    • Women without a partner.<\/li>\n<\/ul>\n[\/vc_column_text][\/vc_column][\/vc_row][vc_row css=”.vc_custom_1617314642126{padding-top: 0px !important;padding-bottom: 0px !important;}”][vc_column width=”1\/2″][vc_single_image image=”5750″ img_size=”400×400″ alignment=”center”][\/vc_column][vc_column width=”1\/2″][vc_column_text]\n

      How is IVF done?<\/h2>\n

      The woman is given medication to stimulate her ovaries<\/strong> and produce many ovarian follicles. Then, at the appropriate time, the woman is ovulated<\/strong>, followed by fertilisation of the eggs from the sperm sample in the laboratory by the embryologists.<\/p>\n

      The fertilised eggs are cultured in appropriate culture media and grow into embryos<\/strong>. The dishes containing the fertilised eggs (zygotes) are placed in an incubator for 2-5 days under special conditions to allow the embryos to go through the early stages of development<\/strong>.<\/p>\n

      The embryos are then transferred back to the woman’s uterus(embryo transfer<\/strong>) and 12 days after the embryo transfer, a pregnancy test is done to confirm the pregnancy.<\/p>\n[\/vc_column_text][\/vc_column][\/vc_row][vc_row content_placement=”middle” css=”.vc_custom_1617312837202{padding-top: 0px !important;padding-bottom: 0px !important;}”][vc_column width=”1\/2″ el_class=”vc_col-sm-push-6″][vc_single_image image=”5726″ img_size=”400×400″ alignment=”center”][\/vc_column][vc_column width=”1\/2″ el_class=”vc_col-sm-pull-6″][vc_column_text css=”.vc_custom_1749212432356{margin-bottom: 0px !important;}”]\n

      Stimulation of the ovaries<\/h2>\n

      Ovarian stimulation<\/strong> during IVF consists of daily injections that cause the ovaries, instead of producing just one follicle, to produce many more so that a larger number of eggs can be obtained.<\/p>\n

      The treatment lasts from 8 to 12 days, depending on the protocol used and the speed of each patient’s response. During the treatment, a series of 3 or 4 ultrasounds are performed as well as hormone measurements in order to check that there is normal development of the follicles<\/strong> .<\/p>\n[\/vc_column_text][\/vc_column][\/vc_row][vc_row content_placement=”middle” css=”.vc_custom_1617312837202{padding-top: 0px !important;padding-bottom: 0px !important;}”][vc_column width=”1\/2″][vc_single_image image=”5713″ img_size=”400×400″ alignment=”center”][\/vc_column][vc_column width=”1\/2″][vc_column_text css=”.vc_custom_1749212559404{margin-bottom: 0px !important;}”]\n

      Ovulation – What is it and how is it done?<\/h2>\n

      Oocyte retrieval is the process of retrieving eggs from the ovaries<\/strong> . When we have evidence through ultrasound that the follicles have reached a satisfactory size and we consider that there is an appropriate number, oocyte retrieval is scheduled 36 hours after the administration of an injection of chorionic gonadotropin, which causes the eggs to mature in a similar way as in a natural cycle.
      \nIt is performed in the operating room under anesthesia, so that the woman does not feel any discomfort during the procedure<\/strong> , which lasts approximately 15 minutes.
      \nEgg retrieval is performed transvaginally<\/strong> , under continuous ultrasound control. The follicles are punctured one by one through a needle that penetrates the vaginal wall. The contents of each follicle are aspirated into special tubes and immediately delivered to the embryology laboratory.
      \nThe embryologist locates the eggs, isolates them from the follicular fluid and places them in special small containers, called dishes, containing a special culture medium. The dishes are then placed in an incubator under the appropriate culture conditions.<\/p>\n[\/vc_column_text][\/vc_column][\/vc_row][vc_row content_placement=”middle” css=”.vc_custom_1617312837202{padding-top: 0px !important;padding-bottom: 0px !important;}”][vc_column width=”1\/2″ el_class=”vc_col-sm-push-6″][vc_single_image image=”5711″ img_size=”400×400″ alignment=”center”][\/vc_column][vc_column width=”1\/2″ el_class=”vc_col-sm-pull-6″][vc_column_text]\n

      Fertilization<\/h2>\n

      After egg retrieval, sperm is collected<\/strong> from the partner and the eggs are fertilized. The fertilization process is carried out either through classic in vitro fertilization where a few thousand sperm are placed around an egg or through intracytoplasmic sperm injection ( ICSI<\/strong> ) where a live sperm is selected and inserted into the egg by the embryologist.
      \nAt this point, it should be mentioned that there are two more new methods that ensure the correct selection and use of the highest quality and functional sperm and improve success rates. These are IMSI<\/strong> and PICSI<\/strong> .
      \nWith the first method, IMSI (intracytoplasmic injection of morphologically selected sperm), the morphologically perfect sperm are selected with a special -high-resolution- microscope (which provides a magnification 6,000 times greater than that in the ICSI method) and are then placed inside the egg.
      \nWith the PICSI method, sperm is selected depending on whether or not it binds to the hyaluronan hydrogel substance, which covers the dish in which we place the sperm. Based on the fact that only normal sperm, with the correct number of chromosomes and a low rate of DNA fragmentation, binds to this substance, the embryologist easily selects the quality sperm and microfertilization follows.
      \nThe day after fertilization, the embryos are “graded”<\/strong> according to their morphological characteristics and their ability to divide. Some may not have developed and are rejected because they are considered non-viable. This correct morphology seems to be associated with spermatozoa that have the correct number of chromosomes and therefore with the creation of embryos that also have the correct number of chromosomes.<\/p>\n[\/vc_column_text][\/vc_column][\/vc_row][vc_row content_placement=”middle” css=”.vc_custom_1617312837202{padding-top: 0px !important;padding-bottom: 0px !important;}”][vc_column width=”1\/2″][vc_single_image image=”7466″ img_size=”400×400″ alignment=”center” css=””][\/vc_column][vc_column width=”1\/2″][vc_column_text]\n

      Embryo transfer<\/h2>\n

      It is the process of transferring the embryos into the uterine cavity.
      \nEmbryo transfer is painless and short, no anaesthesia is required.<\/p>\n

      The best embryos are selected for transfer to the mother’s womb and the embryo transfer is performed with a special catheter.<\/p>\n

      The whole process is done with great care and thoroughness. The woman usually remains lying down for about an hour and then she can return home.<\/p>\n[\/vc_column_text][\/vc_column][\/vc_row][vc_row css=”.vc_custom_1731610336987{padding-top: 40px !important;padding-bottom: 40px !important;}”][vc_column][vc_column_text css=”.vc_custom_1731610322849{margin-bottom: 0px !important;}”]\n

      Read also<\/em><\/h1>\n[\/vc_column_text][\/vc_column][\/vc_row][vc_row css=”.vc_custom_1731610347023{border-bottom-width: 0px !important;padding-top: 0px !important;padding-bottom: 60px !important;}”][vc_column width=”1\/3″][vc_column_text css=”.vc_custom_1731610269248{border-top-width: 2px !important;padding-top: 20px !important;padding-right: 20px !important;padding-bottom: 20px !important;padding-left: 20px !important;background-color: #ffffff !important;border-top-color: #b21059 !important;border-top-style: solid !important;}”]\n

      Poor responders to IVF cycles – Management & better protocols<\/h3>\n

      The incidence rate of poor responders (PORs) to stimulation in IVF cycles currently ranges from 7 to 24%. This rate is even higher in patients aged 38 years and older.[\/vc_column_text][mk_button dimension=”outline” icon=”mk-icon-angle-right” url=”?page_id=5052″ outline_skin=”custom” outline_active_color=”#b21059″ outline_active_text_color=”#b21059″ outline_hover_bg_color=”#b21059″ outline_hover_color=”#ffffff”]MORE[\/mk_button][\/vc_column][vc_column width=”1\/3″][vc_column_text css=”.vc_custom_1731610278851{border-top-width: 2px !important;padding-top: 20px !important;padding-right: 20px !important;padding-bottom: 20px !important;padding-left: 20px !important;background-color: #ffffff !important;border-top-color: #b21059 !important;border-top-style: solid !important;}”]\n

      What does a good number of eggs recovered for IVF mean – What to expect?<\/h3>\n

      In almost all IVF cases, the initial stage involves the ovarian stimulation phase and the subsequent induction of ovulation.[\/vc_column_text][mk_button dimension=”outline” icon=”mk-icon-angle-right” url=”?page_id=5115″ outline_skin=”custom” outline_active_color=”#b21059″ outline_active_text_color=”#b21059″ outline_hover_bg_color=”#b21059″ outline_hover_color=”#ffffff”]MORE[\/mk_button][\/vc_column][vc_column width=”1\/3″][vc_column_text css=”.vc_custom_1731610288705{border-top-width: 2px !important;padding-top: 20px !important;padding-right: 20px !important;padding-bottom: 20px !important;padding-left: 20px !important;background-color: #ffffff !important;border-top-color: #b21059 !important;border-top-style: solid !important;}”]\n

      What is ovarian hyperstimulation syndrome (OHSS)?<\/h3>\n

      Ovarian hyperstimulation syndrome (OHSS) is a complication that can occur with assisted reproductive treatments. It is characterised by an abnormally high ovarian response to drugs given to stimulate the ovaries, especially in in vitro fertilisation (IVF).[\/vc_column_text][mk_button dimension=”outline” icon=”mk-icon-angle-right” url=”?page_id=5135″ outline_skin=”custom” outline_active_color=”#b21059″ outline_active_text_color=”#b21059″ outline_hover_bg_color=”#b21059″ outline_hover_color=”#ffffff”]MORE[\/mk_button][\/vc_column][\/vc_row]\n<\/div>","protected":false},"excerpt":{"rendered":"

      In vitro fertilisation (IVF): how is it done? \u2192 Ovarian stimulation \u2192 Ovulation \u2192 Fertilization \u2192 Fertilization \u2192 Embryo transfer. <\/p>\n","protected":false},"author":3,"featured_media":7466,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"inline_featured_image":false,"footnotes":""},"class_list":["post-4397","page","type-page","status-publish","has-post-thumbnail","hentry"],"yoast_head":"\nIn Vitro Fertilization (IVF) | In Vitro Fertilization<\/title>\n<meta name=\"description\" content=\"In vitro fertilisation (IVF):In which cases is it recommended? 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